![]() Ghanem A, Healey R, Adly FG (2013) Current trends in separation of plasmid DNA vaccines: a review. Liu MA (2011) DNA vaccines: an historical perspective and view to the future. Pereira LR, Prazeres DMF, Mateus M (2010) Hydrophobic interaction membrane chromatography for plasmid DNA purification: design and optimization. Sousa A, Sousa F, Queiroz JA (2012) Advances in chromatographic supports for pharmaceutical-grade plasmid DNA purification. This behaviour can be linked to the presence of the more hydrophobic phenyl group in Capto Adhere, leading to stronger retention of ssDNA molecules, which have a more hydrophobic character due to a higher degree of base exposure. Another pronounced difference between the resins was observed in the inverted elution of ss- and dsDNA, where ssDNA eluted at 2.88 M NaCl on Capto Adhere, while on Capto Q ImpRes ssDNA eluted already at 1.47 M NaCl. This recognition was not observed for Capto Adhere. Capto Q ImpRes provided a recognition for guanylate bases when samples of deoxynucleotides or poly(dG) were examined. All deoxynucleotides and DNAs tested bound strongly to the chromatographic materials and could be eluted by a linear gradient of increasing NaCl concentration. These variations in biophysical properties have been utilized for comparative separations on these two resins. The intrinsic differences between single- and double-stranded DNAs are related to charge, hydrophobicity, size and three-dimensional structure. Capto Adhere carries a multimodal ligand which combines strong anion with aromatic recognition, while Capto Q ImpRes is a strong anion exchanger with a chemically similar ligand, but without a phenyl group. ![]() Stable in up to 1 M NaOH, 40% Isopropanol, up to 1 M acetic acid, and up to 70% ethanol.Ģ0% ethanol at temperatures between 4☌ and 30☌.The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere and Capto Q ImpRes. Hydrophilic properties of base matrix prevent non-specific binding.īioProcess resin supported for industrial applications.Ĭonvenient small-scale purification, process development, and scale-up using prepacked HiTrap columns. Higher throughput for improved productivity and process economy. ![]() High-flow purification of supercoiled DNA from research scale to cGMP production.įlexible process design due to a large operational window of flow velocities and bed heights. Capto PlasmidSelect is available in bulk as well as in formats suitable for process development. The possibility to run at higher flow rates and bed heights increases flexibility in process design and allows for an increased productivity. Compared with its predecessor PlasmidSelect Xtra resin, Capto PlasmidSelect is based on a more rigid base matrix, delivering excellent pressure-flow properties to plasmid production. The resin is designed for purification of supercoiled DNA to high quality for gene therapy and DNA vaccine applications. ![]() Capto PlasmidSelect is a chromatography resin that allows supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.Ĭapto PlasmidSelect is a thiophilic aromatic adsorption chromatography resin with a selectivity that allows supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms. ![]()
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